Catalog Number - 64408-25P

Nova Similars C8™ Octyl is a traditional reversed-phase HPLC stationary phase manufactured on high-purity Type-B spherical silica with a fully endcapped octyl (C8) bonded phase. Designed as a cost-effective alternative to Waters™ Nova-Pak® C8 columns, it offers comparable chromatographic behavior for USP L7 methods while providing the moderate hydrophobic retention and reproducibility expected from a conventional C8 stationary phase. Compared to C18 columns, C8 phases generally provide reduced retention and faster elution of hydrophobic analytes.

64408-25P is a 250 mm x 4.6 mm ID analytical column packed with 4 µm Nova Similars C8 particles. The extended 250 mm length provides increased theoretical plate counts and enhanced resolving power for impurity profiling, complex sample matrices, and challenging separations where maximum resolution is required. The 4 µm particle size provides improved efficiency and somewhat sharper peaks than comparable 5 µm packings while maintaining moderate operating pressure on conventional HPLC systems. Typical operating flow rates are approximately 1.0–2.0 mL/min.

This USP L7 column is ideal for hydrophobic and neutral compounds when less retention is desired than a comparable C18 phase. Common applications include pharmaceutical analysis, long-chain fatty acid separations, impurity testing, and method development. This configuration remains the most popular Nova Similars C8 column in the product line.

Key Benefits

• Type-B spherical silica stationary phase

• Suitable alternative for USP L7 methods

• Comparable selectivity to Waters™ Nova-Pak® C8 columns

• Increased resolution from 250 mm column length

• Higher efficiency than comparable 5 µm columns

• Lower retention than C18 columns

• Excellent for hydrophobic and neutral compounds

• Most popular Nova Similars C8 column configuration

Typical Applications

• USP L7 methods

• Reversed-phase HPLC

• Pharmaceutical analysis

• Impurity profiling

• Method development

• Long-chain fatty acid analysis

• Quality control testing

• Method transfer and validation

Specifications

• Product Type: HPLC Column

• Stationary Phase: Nova Similars C8 Octyl

• USP Classification: L7

• Particle Size: 4 µm

• Particle Type: Type-B Spherical Silica

• Bonded Phase: C8 (Octyl)

• Endcapped: Yes

• Column Dimensions: 4.6 mm ID x 250 mm

• Length: 250 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• Longer column length provides increased resolving power

• Higher efficiency than comparable 5 µm columns

• Moderate operating pressure on conventional HPLC systems

• Provides less retention than comparable C18 stationary phases

• Excellent alternative for many USP L7 methods

• Most popular Nova Similars C8 column configuration

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